Pathogenic infection confounds induction of the estrogenic biomarker vitellogenin in rainbow trout.
نویسندگان
چکیده
To examine the behavior of the estrogenic biomarker vitellogenin (VTG) under the combined impact of estrogens and pathogens, parasite-infected or noninfected rainbow trout were exposed to two doses of 17β-estradiol (E2). Infected and E2-exposed fish showed significantly lower hepatic VTG mRNA levels than healthy fish. Transcriptome data suggest that this was due to energetic constraints. Reduced responsiveness of the VTG biomarker in parasitized fish might obscure detection of low-level field exposure.
منابع مشابه
Effects of 4-n-nonylphenol and tamoxifen on sGnRH, Estrogen receptor and Vitellogenin gene expression in juvenile rainbow trout
gene expression in juvenile rainbow trout. Short title: Effects of 4-n-nonylphenol and tamoxifen on gene expression in trout. Summary Alkylphenols such as nonylphenol (NP) are one of a wide variety of environmental chemicals reported to have estrogenic effects in both in vitro and in vivo studies. Induction of hepatic vitellogenin (Vg) gene expression is widely used as a biomarker for xenoestro...
متن کاملQuantification of rainbow trout (Oncorhynchus mykiss) zona radiata and vitellogenin mRNA levels using real-time PCR after in vivo treatment with estradiol-17 beta or alpha-zearalenol.
Estrogen receptor-mediated induction of zona radiata (ZR) and vitellogenin (VTG) mRNA and protein in rainbow trout (Oncorhynchus mykiss) was compared to assess their utility as biomarkers for exposure to estrogenic compounds. Partial sequences of rainbow trout ZR and beta-actin were cloned by reverse transcriptase polymerase chain reaction (RT-PCR) using degenerate primers based on conserved re...
متن کاملEvaluation of estrogenic activities of aquatic herbicides and surfactants using an rainbow trout vitellogenin assay.
Estrogenic potencies of four herbicides (triclopyr, 2,4-dichlorophenoxyacetic acid (2,4-D), diquat dibromide, glyphosate), two alkylphenol ethoxylate-containing surfactants (R-11 and Target Prospreader Activator (TPA)), and the binary mixture of surfactants with the herbicides were evaluated using an in vivo rainbow trout vitellogenin assay. Juvenile rainbow trout exposed to 2,4-D (1.64 mg/l) f...
متن کاملEFFECTS OF DIFFERENT TOXICANTS ON REPRODCUCTION OF DIFFERENT FISH Exposure to environmental estrogenic alkyl phenolic chemicals in rainbow trout resulted in a decreased fertility and egg production in females, reduction of gonadal size (Jobling
Exposure to environmental estrogenic alkyl phenolic chemicals in rainbow trout resulted in a decreased fertility and egg production in females, reduction of gonadal size (Jobling et al., 1996; Ashfield et al., 1998), reduced sperm production and demasculinisation of genetic male Japanese medaka, intersex gonads (Nimrod and Benson, 1998; Gimeno et al., 1998) etc. Another frequently observed effe...
متن کاملA role of metallothionein in zinc regulation after oestradiol induction of vitellogenin synthesis in rainbow trout, Salmo gairdneri.
The regulation of metallothionein (MT) biosynthesis in rainbow-trout liver was studied after a single intraperitoneal injection of oestradiol-17 beta. Sampling was performed after 2, 7, 14, 21, 28 and 35 days. Following induction of vitellogenin synthesis in the liver, liver somatic index (LSI) rose from 1.25 to 2.00 in 14 days. Associated with the increase in LSI was an elevation of hepatic vi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Environmental toxicology and chemistry
دوره 31 10 شماره
صفحات -
تاریخ انتشار 2012